4 edition of Neonatal rat heart cells cultured in simulated microgravity found in the catalog.
Neonatal rat heart cells cultured in simulated microgravity
Published
1994
by National Aeronautics and Space Administration, National Technical Information Service, distributor in [Washington, DC, Springfield, Va
.
Written in
Edition Notes
| Statement | Robert E. Akins ... [et al.]. |
| Series | NASA TM -- 111146., NASA technical memorandum -- 111146. |
| Contributions | Akins, Robert E., United States. National Aeronautics and Space Administration. |
| The Physical Object | |
|---|---|
| Format | Microform |
| Pagination | 1 v. |
| ID Numbers | |
| Open Library | OL17793337M |
| OCLC/WorldCa | 34639466 |
Growth of Rauscher cells in simulated microgravity. Ideal conditions for Rauscher cell growth in the RWV were determined and C.R. Hartzell, Neonatal rat heart cells cultured in simulated microgravity. In Vitro Cellular & Developmental Biology. Animal, (5): p, Chopra, V., T.V. Dinh, and E.V. Hannigan, Three-dimensional. Taking Cells to Space Previous research using ground-based simulated microgravity—achieved by placing the CPCs in a 2D rotating culture device—yielded several indicators suggesting microgravity may hold promise for adaptation of CPCs for human therapies on Earth, said Jonathan Baio, who worked as a doctoral student in Kearns-Jonker’s lab at the time of the team’s ISS .
For the Cardiac Stem Cells investigation, researchers cultured human heart stem cells, or cardiovascular progenitor cells (CPCs), aboard the space station. These immature heart cells can develop into several different types of cardiovascular cells and produce greater numbers of them. Baio and team simulated microgravity for 6–7 days on NASA’s International Space Station and cultured neonatal cardiac progenitor cells for 12 days in .
Compared to real microgravity in space, simulated microgravity seems to offer some advantages for stem cell culture and differentiation. Due to the fact that—especially in the RWV-type bioreactors—the cells are in permanent free fall through a constantly flowing medium, nutrient and growth factor availability is not limited by diffusion rates. American Journal of Physiology Heart and Circulatory Physiology Publication Venue For Understanding sex differences in long-term blood pressure regulation: insights from experimental studies and computational modeling.
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In Vitro Cell Dev Biol Anim. May;33(5) Neonatal rat heart cells cultured in simulated microgravity. Akins RE(1), Schroedl NA, Gonda SR, Hartzell CR. Collaborators: Akins RE(2).
Author information: (1)Department of Medical Cell Biology, Nemours Research Programs, Alfred I. duPont Institute, Wilmington, Delawareby: Neonatal rat heart cells cultured in simulated microgravity Article (PDF Available) in In Vitro Cellular & Developmental Biology - Animal 33(5) June with 26 Reads How we measure 'reads'.
Get this from a library. Neonatal rat heart cells cultured in simulated microgravity. [Robert E Akins; United States. National Aeronautics and Space Administration.;].
In vitro characteristics of cardiac cells cultured in simulated microgravity are reported. Tissue culture methods performed at unit gravity constrain cells to propagate, differentiate, and interact in a two-dimensional (2D) plane. Neonatal rat cardiac cells in 2D culture organize predominantly as bundles of cardiomyocytes with the intervening areas filled by nonmyocyte cell by: NEONATAL RAT HEART CELLS CULTURED IN SIMULATED MICROGRAVITY ROBERT E.
AKINS,' NANCY A. SCHROEDL, STEVE R. GONDA, AND CHARLES R. HARTZELL Department of Medical Cell Biology, Nemours Research Programs, P0. BoxAlfred I. In vitro characteristics of cardiac cells cultured in simulated microgravity are reported.
Tissue culture methods performed at unit gravity constrain cells to propagate, differentiate, and interact in a two-dimensional (2D) plane. Neonatal rat cardiac cells in 2D culture organize predominantly as bundles of cardiomyocytes with the intervening.
In vitro characteristics of cardiac cells cultured in simulated microgravity are reported. Tissue culture methods performed at unit gravity constrain cells to propagate, differentiate, and interact in a two dimensional (2D) plane. Neonatal rat cardiac cells in 2D culture organize predominantly as bundles of cardiomyocytes with the intervening.
In this study, 3D multi-cellular heart tissue-equivalent model was constructed by culturing neonatal rat myocardial cells in alginate microbeads for one week. With this model we studied the simulated microgravity effects on myocardiocytes by incubat-ing the microbeads in NASA rotary cell culture system with a rate of 15rpm.
Houston, TX, USA – S paceflight changes much about the human body, including how the heart functions and how cells that create heart tissue behave. Scientists studying these changes on the International Space Station continue to report important discoveries.
For the Cardiac Stem Cells investigation, researchers cultured human heart stem cells, or cardiovascular progenitor cells (CPCs. Primary rat neonatal cardiomyocytes were placed into simulated microgravity (μg)or normal gravity (1xg) conditions (Fig. 1A). High-resolution nanoscale liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed as outlined in Fig.
1Bon cells isolated over time at 12, 48, or h from μgor 1xgconditions. Figure 1. Satellite cells are postnatal myoblasts responsible for providing additional nuclei to growing or regenerating muscle cells. Satellite cells retain the capacity to proliferate and differentiate in vitro and, therefore, provide a useful model to study postnatal muscle development.
Most culture systems used to study postnatal muscle development are limited by the two-dimensional (2-D) confines. The growth of cells in NASA bioreactors has been shown to produce: [1] Mineralization, type I collagen biosynthesis, and three-dimensional growth in human and rat osteosarcoma cell lines (Gonda et al., ; ); [2] Formation of contracting myotubes in satellite cells cultured on Matrigel-coated beads (Molnar et al., ).
Primary rat neonatal cardiomyocytes were placed into simulated microgravity (μg) or normal gravity (1xg) conditions (Fig. 1A). High-resolution nanoscale liquid chromatography. Charles R. Hartzell's 43 research works with 1, citations and reads, including: Cardiac Organogenesis in Vitro: Reestablishment of Three-Dimensional Tissue Architecture by Dissociated.
Neonatal rat heart cells cultured in simulated microgravity. In Vitro Cell. Dev. Biol. Anim. ; View in Article. For the Cardiac Stem Cells investigation, researchers cultured human heart stem cells, or cardiovascular progenitor cells (CPCs), aboard the space station.
These immature heart cells can. Neonatal rat heart cells were seeded in hydrogel and cultured on collagen sponge scaffolds either statically or in bioreactors in two different media (control, CTL, or with supplemental growth factor, IGF-I).
Neonatal Rat Heart Cells Cultured in Simulated Microgravity. Akins RE, Schroedl NA, Gonda SR and Hartzell CR: In Vitro Cell Dev Biol Anim[Abstract]. NASA-TM-U I CO Neonatal Rat Heart Cells Cultured in Simulated Microgravity Robert E. Akins1, Nancy A. Schroedl1, Steve R.
Gonda2, and Charles R. Hartzell1 1 Department of Medical Cell Biology, Nemours Research Programs, Alfred I. duPont Institute, Wilmington, DE 3D culture and microgravity, a condition in which objects appear to be weightless, can profoundly modulate cell proliferation and survival.
3D culture allows cells to self-organize by aggregation. The presence of microgravity conditions deeply affects the human body functions at the systemic, organ and cellular levels. This study aimed to investigate the effects induced by simulated-microgravity on non-stimulated Jurkat lymphocytes, an immune cell phenotype considered as a biosensor of the body responses, in order to depict at the cellular level the effects of such a peculiar condition.In vitro studies have demonstrated that space flight and simulated microgravity induce significant changes in gene expression patterns, autophagy, 27 cell migration, 28, 29 extracellular matrix composition 30 and the cytoskeleton.
31 The clinostat is widely used for space biology research, as it can simulate the effect of microgravity on.The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteoblastic cells.
Using the model of murine MC3T3-E1 osteoblast cells, cellular morphology.
